Dr. Juliane Soukup
(402) 280-3265
jksoukup@creighton.edu

Dr. Soukup's webpage

B.S. Chem., Creighton University - 1993
Ph.D., University of Nebraska Medical Center - 1997

Juliane Soukup, PhD

Professor, Biochemistry

I have a general interest in my laboratory in nucleic acid structure and function. We are investigating both natural and in vitro selected RNA and DNA molecules. Utilizing Nucleotide Analog Interference Mapping (NAIM) we are investigating the important functional groups within ribozymes, deoxyribozymes and riboswitches that are needed for the activity of these molecules.

In order to identify direct contacts between riboswitches and their ligands we have utilized Nucleotide Analog Interference Suppression (NAIS) in a unique way. In our studies NAIS combines the use of ligand (riboswitch metabolite) analogs with interference modification to identify sites where modifications that previously caused interference with the normal ligand are tolerated with ligand analogs.  These studies have allowed us to determine essential contacts between riboswitches and their ligands.

Recent publications (note J.K. Strauss and J.K. Strauss-Soukup are Dr. Soukup)

Jansen JA, McCarthy TJ, Soukup GA, and Soukup JK. “Backbone and nucleobase contacts to glucosamine-6-phosphate in the glmS ribozyme.”  Nat. Struct. Mol. Biol. 13:  517-52, 2006.

McCarthy, TJ, Plog, MA, Floy, SA, Jansen, JA, Soukup, JK & Soukup, GA. Ligand requirements for glmS ribozyme self-cleavage.  Chemistry & Biology  12:  1221-1226, 2005.

Soukup, JK and Soukup, GA.  "Riboswitches exert genetic control through metabolite-induced conformational change."  Curr. Opin. Struct. Biol.  14:  344-349, 2004.

Soukup, JK, Minakawa, N, Matsuda, A, and Strobel, SA.  "Identification of A-Minor Tertiary Interactions within a Bacterial Group I Intron Active Site by 3-Deazaadenosine Interference Mapping."  Biochemistry  41:  10426-10438, 2002.

Schmeing TM, Seila AC, Hansen JL, Freeborn B, Soukup JK, Scaringe SA, Strobel SA, Moore PB, and Steitz TA.  “A pre-translocational intermediate in protein synthesis observed in crystals of enzymatically active 50S subunits.”  Nat. Struct. Biol. 9:  225-30, 2002.

Strauss-Soukup, J.K. and Strobel, S.A. Ribozyme Enzymology. In: RNA. Söll, D., Nishimura, S., and Moore, P.B. (eds.), Elsevier Science Ltd., Oxford, England, 2001. (Book Chapter)

Strauss-Soukup, J.K. and Strobel, S.A. "Chemical Phylogeny of Group I Introns Based upon Interference Mapping of a Bacterial Ribozyme." J. Mol. Biol., 302: 339-358, 2000.

Strauss-Soukup, J.K. and Strobel, S.A. "Ribozymes: Enzymology." In: Prebiotic Chemistry, Molecular Fossils, Nucleosides, and RNA. Söll, D. ,
Nishimura, S., and Moore, P.B. (eds.), Elsevier Science Ltd., Oxford, England, 1999. (Book Chapter)

Basu, S., Rambo, R.P., Strauss-Soukup, J., Cate, J.H., Ferré-D'Amare, A. R., Strobel, S.A., and Doudna, J.A. "A Specific Monovalent Metal Ion Integral to the A-A Platform of the RNA Tetraloop Receptor." Nature Struc. Biol., 5: 986-992, 1998.

Strauss-Soukup, J.K., Rodrigues, P. and Maher, L.J. "Effect of Base Composition on DNA Bending by Phosphate Neutralization." Biophy. Chem., 72: 297-306, 1998.

Tomky, L., Strauss-Soukup, J.K. and Maher, L.J. "Effects of Phosphate Neutralization on the Shape of the AP-1 Transcription Factor Binding Site in Duplex DNA." Nuc. Acids Res., 26: 2298-2305, 1998.

Strauss-Soukup, J.K., and Maher, L.J. "Electrostatic Effects in DNA Bending by GCN4 Mutants." Biochemistry, 37: 1060-1066, 1998.

Strauss-Soukup, J.K., and Maher, L.J. "Role of Asymmetric Phosphate Neutralization in DNA Bending by PU.1" J. Biol. Chem., 272: 31570-31575, 1997.

Strauss-Soukup, J.K. and Maher, L.J. "DNA Bending by GCN4 Mutants Bearing Cationic Residues." Biochemistry, 36: 10026-10032, 1997.

Strauss-Soukup, J.K., Vaghefi, M.M., Hogrefe, R.I., and Maher, L.J. "Effects of Neutralization Pattern and Stereochemistry on DNA Bending by
Methylphosphonate Substitutions." Biochemistry, 36: 8692-8698, 1997.

Strauss, J.K., Roberts, C., Nelson, M.G., Switzer, C., and Maher, L.J. "DNA Bending by Hexamethylene Tethered Ammonium Ions." Proc. Natl. Acad. Sci. U.S.A., 93: 9515-9520, 1996.

Strauss, J.K., Prakash, T.P., Roberts, C., Switzer, C., and Maher, L.J. "DNA Bending by a Phantom Protein." Chemistry & Biology, 3: 671-678, 1996.

Strauss, J.K. and Maher, L.J. "Asymmetric Phosphate Neutralization and DNA Bending." In: Biological Structure and Dynamics, vol. 2. Sarma, R.H. and Sarma, M.H. (eds.), Adenine Press, Schenectady, NY, 1996. (Book Chapter)

Strauss, J.K. and Maher, L.J. "DNA Bending by Asymmetric Phosphate Neutralization." Science, 266: 1829-1834, 1994.