Assistant Professor, Biochemistry
B.S. University of Northern Iowa (2006)
M.S. University of Iowa (2009)
Ph.D. University of Iowa (2013)
My Research Interests Are:
Research in the Dieckman lab focuses on the mechanisms by which errors in DNA replication and repair and chromatin organization lead to genome instability and disease. Specifically, our main goal is to characterize the interactions of key factors that are essential for the interplay between genomic and epigenetic stability. Two of these factors include the proteins PCNA (the processivity factor that stimulates DNA polymerases during DNA replication and repair) and CAF-1 (the main histone chaperone that is necessary for packaging DNA into nucleosomes following replication and repair). The interaction between PCNA and CAF-1 is crucial for proper DNA synthesis, assembly of nucleosomes, and preservation of epigenetic marks. Students will learn biochemical techniques used in modern biomedical research in order to determine the mechanistic basis of the network of interactions between CAF-1, PCNA, DNA, and other factors during replication-dependent nucleosome assembly and to work towards an understanding of proper DNA maintenance.
- Kondratick CM, Miller, HK, Dieckman, LM, Litman, JM, Washington, MT. “Structures of Proliferating
Cell Nuclear Antigen (PCNA) Mutant Proteins That Are Defective in Establishing Silent Chromatin.” In preparation.
- Kondratick CM, Nguyen, VQ, Dieckman, LM, Boehm, EM, Powers, KT, Washington, MT. “Random Mutations in a Region of the Subunit Interface of PCNA That Is Implicated in Translesion Synthesis and Mismatch Repair.” In preparation.
- Pryor JM, Dieckman LM, Boehm EM, Washington, MT. “Eukaryotic Y-Family Polymerases: A Biochemical and Structural Perspective.” Nucleic Acids and Molecular Biology (Springer). 2014;30:85-108.
- Dieckman LM, Boehm EM, Hingorani MM, Washington MT. “Distinct Structural Alterations in PCNA Block DNA Mismatch Repair.” Biochemistry. 2013 Aug 20;52(33):5611-5619.
- Dieckman LM and Washington, MT. “PCNA Trimer Instability Inhibits Translesion Synthesis by DNA Polymerase Eta and by DNA Polymerase Delta.” DNA Repair. 2013 May 1;12(5):367-376.
- Dieckman LM, Freudenthal BD, Washington MT. “PCNA Structure and Function: Insights from Structures of PCNA Complexes and Post-translationally Modified PCNA.” Subcell Biochemistry (Springer). 2012;62:281-99.
- Dieckman LM, Johnson RE, Prakash S, Washington MT. “Pre-steady State Kinetic Studies of the Fidelity of Nucleotide Incorporation by Yeast DNA Polymerase Delta.” Biochemistry. 2010 Aug 31;49(34):7344-50.